Junior Specialist – Turnbaugh Lab
The Turnbaugh lab is seeking a Junior Specialist to join our group and contribute to new and ongoing projects. This work will focus on the study of the microbiome using Gnotobiotic mice, and will include performing advanced experimental techniques to execute experiments (for example, the Specialist may administer experimental agents via oral, i.p., or subcutaneous routes, and follow up with experimental disease scoring and tracking, dissection and processing of tissues, or other techniques deemed experimentally necessary). Lab techniques performed by the Specialist include (but are not limited to): DNA extraction, PCR, qPCR, microbial cultures, gram staining, and genotyping. The candidate will also be expected to contribute to novel projects advancing research and development of Gnotobiotic tools. For example, the candidate will work on the development of novel models (such as allergy and asthma) in sterile mice and the translation of embryo-transfer techniques into a germ-free setting, among other projects. An exceptional candidate will move these projects forward independently, helping the lab to develop and test new ideas. Additionally, the specialist will be expected to assist with managing day-to-day lab operations within the Gnotobiotic core facility, perform general lab duties, and be a positive lab citizen.
Required Qualifications:
• Excellent organizational and interpersonal communication skills (verbal and written)
• Willingness and ability to learn new methods and skills for changing research priorities.
• Ability to work independently and as a member of a research team.
• Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines.
• Must have (or be in process of obtaining) a bachelor’s degree (or equivalent degree) or at least four years of research experience to be appointed at the Junior rank.
Preferred Qualifications:
• Prior experience with handling pathogenic microbes, aseptic techniques
• Prior experience working in a research-based setting with murine models
• Prior experience in gnotobiotic mouse handling/maintenance in positive pressure isolators, with a focus on sterility
• Prior experience in advanced techniques in murine models (such as injection of compounds, oral gavage, anesthesia, surgery techniques and documentation)
We are seeking at least a 1-year commitment. Depending on performance and workload, opportunities for independent research, mentorship of other trainees, and scientific writing will be available.
Applicants must meet requirements by the time of appointment.
Please apply online at https://aprecruit.ucsf.edu/JPF04523.
Reviewed: 5/24/23
Junior Specialist - Fraser Lab
The Fraser Lab in the UCSF Department of Bioengineering and Therapeutic Sciences have a Junior Specialist position available immediately. Salary will be commensurate with level of experience. The department is focused on developing new areas of therapeutic engineering, pharmacogenomics, computational biology, systems pharmacology, drug development and regulatory sciences. The Fraser lab uses a combination of structural biology, biochemistry, and deep mutational scanning methods for studying how proteins work in physiology and disease. We seek highly motivated and talented candidates interested in high throughput sequencing-based approaches to studying receptor biophysics and biochemistry.
The successful candidate will participate in multiple research projects dependent on skills and interests.
This full-time position will include:
- Administrative and laboratory management duties.
- Performing molecular biology, DNA sequencing, mammalian cell culture, flow cytometry, fluorescence microscopy, and data analysis.
- Working collaboratively on a range of exciting projects The position is for at least two years and ideal for an individual looking to gain additional research experience prior to further development such as graduate, medical, or MD/PhD training
Required qualifications are:
- Bachelors degree prior to start point for appointment to the Junior rank.
- Familiarity with wet lab methods
- Excellent communication
- Proven ability to work as part of a team and an enthusiasm for science
See Table 24B for the salary range for this position. A reasonable estimate for this position is $49,000-$52,200.
Please apply online at https://aprecruit.ucsf.edu/JPF04520 with a CV.
Reviewed: 5/19/23
Junior Specialist – Erlebacher Lab
The Junior Specialist will work in a basic/translational science laboratory on the UCSF Parnassus Campus that focuses on immunological and epigenetic features of pregnancy including why the fetus is not rejected by the maternal immune system, how labor induction is timed and controlled, and mechanisms of pregnancy complications (adrianerlebacherlab.org). Under the supervision of the PI, s/he will work independently on multiple projects and perform a variety of techniques, including:
1) Immunohistochemical and immunofluorescence staining of tissue sections, including photomicroscopy
2) Molecular biology techniques including RNA extraction, PCR, qPCR, RT-PCR, western blots, and PCR primer design using online programs
3) Cell culture, including cell transfections and treatments, and lentivirus preparation
4) Mouse colony maintenance, including tailing mice for genotyping, weaning of mouse litters, preparation of tail DNA and PCR analysis, and record keeping
5) Isolation of cells from mouse tissues, and their analysis by a variety of techniques, including flow cytometry and qRT-PCR.
The applicant must be able to perform detail-oriented tasks, manage multiple tasks at any one time, prioritize and perform experiments with minimal supervision, and have excellent organizational and communication skills.
Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service.
Required Experience, Skills:
Bachelor Degree in science or related majors, as well as familiarity with basic molecular biological, biochemical, and microbiological techniques. Candidates must meet the qualifications by the time of appointment. Candidate’s CV or cover letter must state required degree (or in process) upon submission.
Please apply online at https://aprecruit.ucsf.edu/JPF04041 with a CV, cover letter, and two references.
Reviewed: 5/3/23
Junior Specialist – Chorba Lab
The Chorba Lab seeks a highly motivated Junior Specialist to work as a research technician and laboratory administrator. Under direct supervision and training, the Junior Specialist will work on several highly collaborative projects including 1) the role and druggability of the processing and trafficking pathways of PCSK9 in regulating the LDL receptor, 2) the characterization of CSDE1 as a regulator of LDLR mRNA decay, and 3) the development of methods to chemically perturb protein translation in a sequence specific manner. The incumbent for this position will have many duties relevant to these goals, including the generation of DNA constructs, generation and maintenance of cell lines, and production and purification of recombinant proteins, in addition to standard cell culture and molecular biology techniques. Responsibilities also include assisting with all routine laboratory maintenance (restocking of supplies, assisting with monitoring freezers, and additional duties as necessary). Notably, the successful candidate may be required to attend to cell culture samples or experimental procedures at off-hours or on weekends when necessary.
The successful candidate for this position will have a background in general biology, molecular biology, cell biology, genetics, and chemistry. They will be involved in analyzing the effects of chemical and biochemical perturbations of specific proteins both in vitro and in cells. This work will require a number of biochemical and biologic techniques including (but not limited to): PCR; DNA and protein gel electrophoresis; Western blotting; standard cell culture; flow cytometry; transfections; DNA/RNA extractions; protein purification; buffer preparation; and microplate assays. The candidate must have clear knowledge of molarity, sterile technique, standard Mendelian genetic inheritance, general microbiology, and the central dogma of molecular biology. Specialists appointed at the junior rank must possess a baccalaureate degree (or equivalent degree) or at least four years of relevant research experience (e.g., with instrumentation and research equipment, social science research methods, or creative activities).
Our research efforts are focused on identifying and validating drug targets and developing novel therapeutic strategies to treat cardiovascular disease, with a particular focus on regulators of lipid metabolism and cholesterol levels. To this end, we pursue the molecular mechanisms of how key players in these pathways interact, and how their dysregulation leads to disease. Our work bridges the interface of chemistry and biology, where we develop cutting-edge chemical, biochemical, and genetic tools to pursue our inquiries. We are an inclusive environment and are not afraid to think big.
Please apply online at https://aprecruit.ucsf.edu/JPF03766 or https://aprecruit.ucsf.edu/JPF04472. Applicants’ materials must list current and/or pending qualifications upon submission.
Reviewed: 4/30/23
Junior Specialist - Cyster Lab
The Cyster lab is seeking a Junior Specialist to join our group and contribute to new and ongoing projects. Our lab focuses on the organizer cues and cell migration dynamics underlying adaptive immune responses. We are also interested in defining the signaling requirements for mounting antibody responses. The candidate will be expected to contribute to the study of B cell responses at the molecular, cellular and tissue level using a range of immunology techiques. The candidate will also participate in helping with lab management, including helping with mouse genotyping and colony maintenance, assist with managing day-to-day lab operations, perform general lab duties and help with regulatory compliance. An exceptional candidate will lead their own independent project, develop new ideas, be familiar with the literature, and participate in procurement of funds, where appropriate. The specialist is expected to be a positive lab citizen.
Required Qualifications:
• Excellent organizational and interpersonal communication skills (verbal and written)
• Willingness and ability to learn new methods and skills for changing research priorities.
• Ability to work independently and as a member of a research team.
• Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines.
• Must have (or be in process of obtaining) a bachelor’s degree (or equivalent degree) or four years of research experience to be appointed at the Junior rank.
• Applicants must meet requirements by the time of appointment.
Preferred Qualifications:
• Prior experience with handling mice and tissue isolation.
• Prior experience with high dimensional flow cytometry
• Prior experience with tissue culture and asceptic technique
• Knowedlge of immunology including B cell biology
We are seeking at least a 2-year commitment. Depending on performance and workload, opportunities for independent research, mentorship of other trainees, and scientific writing will be available.
See Table 24B (https://www.ucop.edu/academic-personnel-programs/_files/2022-23/july-2022-salary-scales/t24-b.pdf) for the salary range for this position. A reasonable estimate for this position is $49,000 - $52,200.
UC San Francisco seeks candidates whose experience, teaching, research, or community service has prepared them to contribute to our commitment to diversity and excellence.
The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
Please apply online at https://aprecruit.ucsf.edu/JPF03811 with a CV and the contact information for 2 references.
Junior Specialist – Toczyski Lab
The Cancer Center is seeking a Junior Specialist. Responsibilities include general lab maintenance, including ordering supplies and upkeep. Performing and designing experiments, with frequent input from Dr. Toczyski and other members of the lab. These will involve both cell biological and biochemical experiments. Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service.
Required Qualifications:
• Specialists appointed at the junior rank must possess a baccalaureate degree (or equivalent degree) in Molecular Biology (or a related field) or must have at least four years of research experience (e.g., with instrumentation and research equipment, social science research methods, or creative activities).
• Undergraduates or work related molecular biology research experience required.
• Incumbent must have experience in lab maintenance, protein sample preparation and western blotting, cloning, protein purification, and tissue culture.
• All requirements must be met by the appointment date.
• Applicant’s materials must list (pending) qualifications upon submission.
Preferred Qualifications:
• Post-graduate training in field.
Please apply online at https://aprecruit.ucsf.edu/JPF04196.
Reviewed: 2/22/23
Junior Specialist - Kroetz Lab
A Junior Specialist position is available in the Kroetz laboratory to investigate the role of nuclear S1P in the effects of microtubule targeting agents on sensory neuron transcriptional networks. The role will involve differentiation of sensory neurons from induced pluripotent stem cells and scRNA- and scATAC-sequencing of sensory neurons treated with microtubule targeting agents. The percent experimental and computational work will vary over the course of the project but will include both roles.
Appointees in the Specialist series will be expected to engage in specialized research, professional activities and do not have teaching responsibilities. Specialists are expected to use their professional expertise to make scientific and scholarly contributions, and may participate in University and Public Service. Screening of applicants will begin immediately and will continue as needed throughout the recruitment period. Salary and rank will be commensurate with the applicants experience and training.
Applicants must have a Bachelor’s degree in biological sciences and some experience with laboratory based research, by time of appointment.
Please apply online at https://aprecruit.ucsf.edu/JPF03932 with a CV, cover letter, and the contact information of 3 references. CV must list pending qualifications.
Reviewed: 7/18/22